Nitrogen vacancy (NV) center fluorescent nanodiamond (FND) suspension in water with 0.01% sodium azide as a preservative. Polyethylene glycol, of length 4, 12, or 36 units, is conjugated to Streptavidin and attached covalently to the diamond surface. NV FNDs are available with 40 or 100 nanometer average diameter.
|Shipped in||50mM HEPES, pH 7.4, 0.05% Tween-20, 0.01% Sodium Azide (available azide-free upon request)|
|Storage Instructions||Store at 4˚C. Do not freeze. Vortex lightly to resuspend.|
|Concentration||200µL at 1mg/mL FND (200µg)|
|Source||Streptavidin (Streptomyces avidinii)|
|Mass||52 KDa, 4 subunit protein|
|Purity||Single band assessed via SDS-PAGE|
|Activity of Streptavidin preparation before conjugation||14U/mg|
Streptavidin NV FND comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Nitrogen Vacancy Fluorescent Nanodiamond, or NV FND) via a PEG linker. Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Cymaris NV FND conjugates are provided as 1 mg/ml solutions.