Streptavidin-Conjugated PEGylated Fluorescent Nanodiamond (FND)

Streptavidin-conjugated nitrogen vacancy (NV) center fluorescent nanodiamonds are formulated with a proprietary biocompatible coating technology. PEG spacers are included to increase streptavidin conjugation and functional activity.

Catalog #
Product
ND Size (nm)

Amount (ug)

Price (USD)








12010
Streptavidin-PEG  4-FND-40
40
200
$280.00
12110
Streptavidin-PEG  4-FND-100
100
200
$200.00
12040
Streptavidin-PEG 12-FND-40
40
200
$280.00
12140
Streptavidin-PEG 12-FND-100
100
200
$200.00
12050
Streptavidin-PEG 36-FND-40
40
200
$280.00
12150
Streptavidin-PEG 36-FND-100
100
200
$200.00








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Product overview:

Nitrogen vacancy (NV) center fluorescent nanodiamond (FND) suspension in water with 0.01% sodium azide as a preservative. Polyethylene glycol, of length 4, 12, or 36 units, is conjugated to Streptavidin and attached covalently to the diamond surface.  NV FNDs are available with 40 or 100 nanometer average diameter.

Properties:
Shipped in
50mM HEPES, pH 7.4, 0.05% Tween-20, 0.01% Sodium Azide (available azide-free upon request)
Storage InstructionsStore at 4˚C. Do not freeze. Vortex lightly to resuspend.
Concentration200µL at 1mg/mL FND (200µg)
SourceStreptavidin (Streptomyces avidinii)
Mass52 KDa, 4 subunit protein
PuritySingle band assessed via SDS-PAGE
Activity of Streptavidin preparation before conjugation
14U/mg


Applications:

Streptavidin NV FND comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Nitrogen Vacancy Fluorescent Nanodiamond, or NV FND) via a PEG linker. Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Cymaris NV FND conjugates are provided as 1 mg/ml solutions.

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*FOR RESEARCH USE ONLY*

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